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non targeting nt control sgrna  (Addgene inc)


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    Structured Review

    Addgene inc non targeting nt control sgrna
    Non Targeting Nt Control Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+targeting+control+sgrna/lentiCRISPR+v2+(Plasmid+%2352961)/pm41840739-171-12-20
    Average 96 stars, based on 6181 article reviews
    non targeting nt control sgrna - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Knockdown:

    Article Title: KSR1- and ERK-dependent Translational Regulation of the Epithelial-to-Mesenchymal Transition
    Article Snippet: .. The KSR1 knockdown was confirmed via Western Blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer
    Article Snippet: .. For OVOL2 CRISPR-interference (CRISPRi) knockdown two sgRNAs (sg1: CACCGTCGCGAGTGAGACCACGCCG, sg2: CACCGTTGACACCGTTATGTTGCA) targeting the OVOL2 promoter and a non-targeting control sgRNA (NT: CTGAAAAAGGAAGGAGTTGA) were cloned in the lentiviral vector Plv-hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (gift from Charles Gersbac (Addgene plasmid # 71,236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236)) [ ] with Esp3I (Thermo Fisher Scientific, USA). ..

    Article Title: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
    Article Snippet: .. The KSR1 knockdown was confirmed via western blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Western Blot:

    Article Title: KSR1- and ERK-dependent Translational Regulation of the Epithelial-to-Mesenchymal Transition
    Article Snippet: .. The KSR1 knockdown was confirmed via Western Blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
    Article Snippet: .. The KSR1 knockdown was confirmed via western blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Plasmid Preparation:

    Article Title: KSR1- and ERK-dependent Translational Regulation of the Epithelial-to-Mesenchymal Transition
    Article Snippet: .. The KSR1 knockdown was confirmed via Western Blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer
    Article Snippet: .. For OVOL2 CRISPR-interference (CRISPRi) knockdown two sgRNAs (sg1: CACCGTCGCGAGTGAGACCACGCCG, sg2: CACCGTTGACACCGTTATGTTGCA) targeting the OVOL2 promoter and a non-targeting control sgRNA (NT: CTGAAAAAGGAAGGAGTTGA) were cloned in the lentiviral vector Plv-hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (gift from Charles Gersbac (Addgene plasmid # 71,236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236)) [ ] with Esp3I (Thermo Fisher Scientific, USA). ..

    Article Title: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
    Article Snippet: .. The KSR1 knockdown was confirmed via western blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Control:

    Article Title: KSR1- and ERK-dependent Translational Regulation of the Epithelial-to-Mesenchymal Transition
    Article Snippet: .. The KSR1 knockdown was confirmed via Western Blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival
    Article Snippet: HEK293T cells were then seeded in 6-well dishes (6×10 5 cells/well) and reverse transfected using Polyfect (Qiagen, 301105) following the manufacturers protocol. .. Transfection was carried out using 880 ng of lentiCRISPR-V2 with respective target-or non-targeting control sgRNA, 600 ng psPAX2 (Addgene, 12260), and 300 ng pMD2 (Addgene, 12259). ..

    Article Title: OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer
    Article Snippet: .. For OVOL2 CRISPR-interference (CRISPRi) knockdown two sgRNAs (sg1: CACCGTCGCGAGTGAGACCACGCCG, sg2: CACCGTTGACACCGTTATGTTGCA) targeting the OVOL2 promoter and a non-targeting control sgRNA (NT: CTGAAAAAGGAAGGAGTTGA) were cloned in the lentiviral vector Plv-hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (gift from Charles Gersbac (Addgene plasmid # 71,236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236)) [ ] with Esp3I (Thermo Fisher Scientific, USA). ..

    Article Title: SETD2 safeguards the genome against isochromosome formation
    Article Snippet: .. HeLa TetOn-Cas9 cells were transduced with 8mg/mL polybrene (Millipore Sigma, TR-1003) and viral supernatant for guides targeting SETD2 or non-targeting control sgRNA (Addgene, 80189). .. Cells were selected using 0.5μg/mL puromycin (Millipore Sigma, P9620).

    Article Title: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
    Article Snippet: .. The KSR1 knockdown was confirmed via western blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: BAP1 deletion abrogates growth and metastasis of murine cutaneous melanoma
    Article Snippet: .. The sequence of non-targeting control sgRNA was from Addgene (#80173). ..

    Article Title: A stress-induced Tyrosine tRNA depletion response mediates codon-based translational repression and growth suppression
    Article Snippet: .. A non-targeting control sgRNA (sequence retrieved from hCRISPRi v2 ( )) and 5 sgRNAs targeting DIS3L2 (generated using the ChopChop tool ( )) were cloned into lentiGuide-Puro (Addgene #52963). .. The individual sgRNAs were subsequently transduced into MCF10a cells stably expressing pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and the cells expressing the sgRNAs were enriched for using 1.2 ug/mL puromycin selection.

    Article Title: Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance
    Article Snippet: The truncated mutant forms of PRDX3 (1–221aa, 63–221aa, 63–256aa) were cloned into the pGEX-4T-1 vector (Adgene, #27-4580-01). .. Clones expressing two non-overlapping sgRNAs directed against human PHB or a non-targeting control sgRNA that has no targets in the human genome were cloned into the LentiGuide-puro vectors (Addgene); Clones expressing PHB DOX-induced shRNAs were cloned into the Tet-pLKO-puro vectors (Addgene); Clones expressing PHB, PRDX3 or EIF4A shRNAs were cloned into the pLKO.1 TRC vectors (Addgene). ..

    Clone Assay:

    Article Title: KSR1- and ERK-dependent Translational Regulation of the Epithelial-to-Mesenchymal Transition
    Article Snippet: .. The KSR1 knockdown was confirmed via Western Blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer
    Article Snippet: .. For OVOL2 CRISPR-interference (CRISPRi) knockdown two sgRNAs (sg1: CACCGTCGCGAGTGAGACCACGCCG, sg2: CACCGTTGACACCGTTATGTTGCA) targeting the OVOL2 promoter and a non-targeting control sgRNA (NT: CTGAAAAAGGAAGGAGTTGA) were cloned in the lentiviral vector Plv-hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (gift from Charles Gersbac (Addgene plasmid # 71,236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236)) [ ] with Esp3I (Thermo Fisher Scientific, USA). ..

    Article Title: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
    Article Snippet: .. The KSR1 knockdown was confirmed via western blotting. pCAG-SpCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid #79144), KSR1 sgRNA and non-targeting control sgRNA was cloned into the pCas9 vector. ..

    Article Title: A stress-induced Tyrosine tRNA depletion response mediates codon-based translational repression and growth suppression
    Article Snippet: .. A non-targeting control sgRNA (sequence retrieved from hCRISPRi v2 ( )) and 5 sgRNAs targeting DIS3L2 (generated using the ChopChop tool ( )) were cloned into lentiGuide-Puro (Addgene #52963). .. The individual sgRNAs were subsequently transduced into MCF10a cells stably expressing pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and the cells expressing the sgRNAs were enriched for using 1.2 ug/mL puromycin selection.

    Article Title: Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance
    Article Snippet: The truncated mutant forms of PRDX3 (1–221aa, 63–221aa, 63–256aa) were cloned into the pGEX-4T-1 vector (Adgene, #27-4580-01). .. Clones expressing two non-overlapping sgRNAs directed against human PHB or a non-targeting control sgRNA that has no targets in the human genome were cloned into the LentiGuide-puro vectors (Addgene); Clones expressing PHB DOX-induced shRNAs were cloned into the Tet-pLKO-puro vectors (Addgene); Clones expressing PHB, PRDX3 or EIF4A shRNAs were cloned into the pLKO.1 TRC vectors (Addgene). ..

    Transfection:

    Article Title: Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival
    Article Snippet: HEK293T cells were then seeded in 6-well dishes (6×10 5 cells/well) and reverse transfected using Polyfect (Qiagen, 301105) following the manufacturers protocol. .. Transfection was carried out using 880 ng of lentiCRISPR-V2 with respective target-or non-targeting control sgRNA, 600 ng psPAX2 (Addgene, 12260), and 300 ng pMD2 (Addgene, 12259). ..

    CRISPR:

    Article Title: OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer
    Article Snippet: .. For OVOL2 CRISPR-interference (CRISPRi) knockdown two sgRNAs (sg1: CACCGTCGCGAGTGAGACCACGCCG, sg2: CACCGTTGACACCGTTATGTTGCA) targeting the OVOL2 promoter and a non-targeting control sgRNA (NT: CTGAAAAAGGAAGGAGTTGA) were cloned in the lentiviral vector Plv-hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (gift from Charles Gersbac (Addgene plasmid # 71,236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236)) [ ] with Esp3I (Thermo Fisher Scientific, USA). ..

    Transduction:

    Article Title: SETD2 safeguards the genome against isochromosome formation
    Article Snippet: .. HeLa TetOn-Cas9 cells were transduced with 8mg/mL polybrene (Millipore Sigma, TR-1003) and viral supernatant for guides targeting SETD2 or non-targeting control sgRNA (Addgene, 80189). .. Cells were selected using 0.5μg/mL puromycin (Millipore Sigma, P9620).

    Sequencing:

    Article Title: BAP1 deletion abrogates growth and metastasis of murine cutaneous melanoma
    Article Snippet: .. The sequence of non-targeting control sgRNA was from Addgene (#80173). ..

    Article Title: A stress-induced Tyrosine tRNA depletion response mediates codon-based translational repression and growth suppression
    Article Snippet: .. A non-targeting control sgRNA (sequence retrieved from hCRISPRi v2 ( )) and 5 sgRNAs targeting DIS3L2 (generated using the ChopChop tool ( )) were cloned into lentiGuide-Puro (Addgene #52963). .. The individual sgRNAs were subsequently transduced into MCF10a cells stably expressing pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and the cells expressing the sgRNAs were enriched for using 1.2 ug/mL puromycin selection.

    Generated:

    Article Title: A stress-induced Tyrosine tRNA depletion response mediates codon-based translational repression and growth suppression
    Article Snippet: .. A non-targeting control sgRNA (sequence retrieved from hCRISPRi v2 ( )) and 5 sgRNAs targeting DIS3L2 (generated using the ChopChop tool ( )) were cloned into lentiGuide-Puro (Addgene #52963). .. The individual sgRNAs were subsequently transduced into MCF10a cells stably expressing pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and the cells expressing the sgRNAs were enriched for using 1.2 ug/mL puromycin selection.

    Expressing:

    Article Title: Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance
    Article Snippet: The truncated mutant forms of PRDX3 (1–221aa, 63–221aa, 63–256aa) were cloned into the pGEX-4T-1 vector (Adgene, #27-4580-01). .. Clones expressing two non-overlapping sgRNAs directed against human PHB or a non-targeting control sgRNA that has no targets in the human genome were cloned into the LentiGuide-puro vectors (Addgene); Clones expressing PHB DOX-induced shRNAs were cloned into the Tet-pLKO-puro vectors (Addgene); Clones expressing PHB, PRDX3 or EIF4A shRNAs were cloned into the pLKO.1 TRC vectors (Addgene). ..



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